Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
J Proteome Res. 2012 Dec 7;11(12):5586-91. doi: 10.1021/pr300426s. Epub 2012 Nov 19.

Recognizing uncertainty increases robustness and reproducibility of mass spectrometry-based protein inferences.

Author information

  • 1Department of Neurobiology, Harvard Medical School Children's Hospital Boston, Boston, Massachusetts, United States. Oliver.Serang@Childrens.Harvard.edu


Parsimony and protein grouping are widely employed to enforce economy in the number of identified proteins, with the goal of increasing the quality and reliability of protein identifications; however, in a counterintuitive manner, parsimony and protein grouping may actually decrease the reproducibility and interpretability of protein identifications. We present a simple illustration demonstrating ways in which parsimony and protein grouping may lower the reproducibility or interpretability of results. We then provide an example of a data set where a probabilistic method increases the reproducibility and interpretability of identifications made on replicate analyses of Human Du145 prostate cancer cell lines.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society Icon for PubMed Central
    Loading ...
    Write to the Help Desk