Biochimie. 1979;61(10):1151-60.

Isolation and identification of mutants constitutive for aspartokinase III synthesis in Escherichia coli K 12.

Abstract

We devised a procedure in order to isolate, in Escherichia coli, constitutive mutants for aspartokinase III synthesis, the first enzyme of the lysine regulon. It consists of the introduction of a limiting step in lysine biosynthesis, by the use of the partial suppression of a nonsense mutation. For the first time we could isolate many constitutive mutants. Their characteristics (cotransduction with the lysC structural gene; no effect on the synthesis of other enzymes of the regulon; cis-dominance) lead to classify these mutations as operator-type. The fact that no repressor mutations could be isolated is discussed.

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Isolation and identification of mutants constitutive for aspartokinase III synthesis in Escherichia coli K 12.
Biochimie. 1979 ;61(10):1151-60.

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