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Nat Methods. 2012 Dec;9(12):1202-5. doi: 10.1038/nmeth.2249. Epub 2012 Nov 11.

Two-photon optogenetics of dendritic spines and neural circuits.

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  • 1Howard Hughes Medical Institute (HHMI), Columbia University, New York, New York, USA.

Abstract

We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions.

PMID:
23142873
[PubMed - indexed for MEDLINE]
PMCID:
PMC3518588
Free PMC Article
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