Expression of cellCAM-105 in the apical surface of rat uterine epithelium is controlled by ovarian steroid hormones

J Reprod Fertil. 1990 Jan;88(1):213-21. doi: 10.1530/jrf.0.0880213.

Abstract

Affinity-purified antibodies to cellCAM-105, an adhesive cell surface glycoprotein, were used in immunohistochemical investigations of rat uteri at various functional stages: (i) the oestrous, pro-oestrous, metoestrous, and dioestrous stages of the oestrous cycle, (ii) Days 1-8 of normal pregnancy, (iii) delayed implantation, (iv) 18 h after oestrogen reactivation from delay of implantation, and (v) juvenile rats, and normal ovariectomized adults, respectively, before and after experimental injection of progesterone and/or oestrogen. CellCAM-105 was present in the apical zones of the luminal and glandular epithelium cells in a stage-specific and hormone-dependent manner. The results indicate that: (1) steroid hormones are essential for the expression of cellCAM-105 in the uterine epithelial cells; (2) progesterone induces cellCAM-105 expression in the glandular epithelium, and oestrogen induces cellCAM-105 expression in the luminal epithelium; (3) progesterone induces down-regulation of cellCAM-105 from the surface of the uterine luminal epithelium of juvenile rats; (4) cellCAM-105 is absent in the luminal epithelial cells but present in the glandular epithelial cells of the rat uterus at the time of blastocyst implantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases*
  • Animals
  • Antigens, CD
  • Cell Adhesion Molecules / metabolism*
  • Embryo Implantation, Delayed / physiology
  • Epithelium / metabolism
  • Estrogens / pharmacology
  • Estrus / metabolism
  • Female
  • Gonadal Steroid Hormones / metabolism*
  • Membrane Glycoproteins*
  • Ovariectomy
  • Pregnancy
  • Progesterone / pharmacology
  • Rats
  • Rats, Inbred Strains
  • Uterus / metabolism*

Substances

  • Antigens, CD
  • CD66 antigens
  • Cell Adhesion Molecules
  • Estrogens
  • Gonadal Steroid Hormones
  • Membrane Glycoproteins
  • Progesterone
  • Adenosine Triphosphatases