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Life Sci. 2012 Dec 17;91(25-26):1323-7. doi: 10.1016/j.lfs.2012.10.015. Epub 2012 Oct 30.

Effects of Rehmannia glutinosa oligosaccharide on human adipose-derived mesenchymal stem cells in vitro.

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  • 1Department of Cardiology, Chinese PLA General Hospital, Beijing 100853, China.



Adipose-derived mesenchymal stem cells (ADMSCs) are considered as a good cell source for regenerative medicine with their self-renew capacity, multilineage differentiation and immunomodulatory potency. Based on this background, the aim of this study was to evaluate the influence of Rehmannia glutinosa oligosaccharide (RGO), a traditional Chinese medicine, on human ADMSCs' proliferation, H(2)O(2)-induced apoptosis, and secretion of Vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in vitro.


Human ADMSCs were isolated and cultured in vitro. Then flow cytometry was carried out to characterize the cells, and MTT assay was performed to detect the proliferation. H(2)O(2)-induced apoptosis was evaluated by flow cytometry. VEGF and HGF production were detected by ELISA kits.


Human ADMSCs were positive for CD90 and CD29, but negative for CD31, CD34 and CD45. The results also indicate that RGO can promote the proliferation and alleviate H(2)O(2)-induced apoptosis of human ADMSCs. The mechanism of RGO's protective effect may involve the up-regulation of VEGF and HGF.


The present study indicates that RGO may increase the viability and proliferative capacity and alleviate H(2)O(2)-induced apoptosis of human ADMSCs via the paracrine release of VEGF and HGF. These results indicate that RGO application will enhance stem cell viability and improve their effects in cell therapy.

Copyright © 2012 Elsevier Inc. All rights reserved.

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