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Food Chem. 2013 Jan 15;136(2):1063-9. doi: 10.1016/j.foodchem.2012.08.092. Epub 2012 Sep 20.

Reverse micellar extraction of bovine serum albumin - a comparison between the effects of gemini surfactant and its corresponding monomeric surfactant.

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  • 1School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, PR China.

Abstract

Gemini surfactant displayed distinct advantages over monomeric surfactant in the liquid-liquid reverse micellar extraction process. First, less amount of gemini surfactant than monomeric surfactant was needed for transferring almost complete bovine serum albumin (BSA) into organic phase from aqueous phase. Second, the loading capacity of gemini surfactant reverse micelle phase was much higher than that of the corresponding monomeric surfactant reverse micelle. Third, efficient backward extraction (75-92%) of BSA could be effected in a wide pH range from 4 to 9 with gemini surfactant reverse micelle while a pH of ca. 4.3 is prerequisite to the recovery of BSA from monomeric surfactant reverse micelle. So far, the reports about the effect of surfactant structure on protein extraction have been limited. This study indicates the important role of the spacer of gemini surfactant in protein extraction process and may provide more knowledge on how to optimise surfactant structure.

Copyright © 2012 Elsevier Ltd. All rights reserved.

PMID:
23122163
[PubMed - indexed for MEDLINE]
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