Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
Cold Spring Harb Protoc. 2012 Nov 1;2012(11):1187-8. doi: 10.1101/pdb.prot072124.

Removing rRNA from deproteinized, phenol-extracted total RNA by enzymatic digestion.


Ribosomal RNAs (28S, 18S, 5.8S, and 5S rRNAs) comprise the vast majority of total cellular RNAs (>95%). When other RNAs are to be analyzed, it is very advantageous to remove the rRNA species. The best way to eliminate rRNA "contamination" is to pellet subunits before deproteinization. When this is not possible, certain RNAs can be separated from bulk rRNA after extraction. This protocol describes enzymatic digestion to remove rRNAs from deproteinized, phenol-extracted total RNA.

[PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Write to the Help Desk