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Wei Sheng Wu Xue Bao. 2012 Jul 4;52(7):902-9.

[Diversity of bacteria isolated from cysts Heterodera glycines in Heilongjiang].

[Article in Chinese]

Author information

  • 1State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. xiaozhu_1688@126.com

Abstract

OBJECTIVE:

To understand the bacterial diversity isolated from the cysts of Heterodera glycines in the soybean field in Heilongjiang Province.

METHODS:

Bacteria were isolated from cysts on nutrient agar plates using dilution plate method and further identified by phylogenetic analysis based on 16S rDNA gene sequences.

RESULTS:

Totally 90 bacteria strains with different colony morphology were selected on nutrient agar plate and their phylogenetic features were analyzed based on the partial 16S rDNA sequences. In total 7 genera and 22 species were identified, including 46 strains in Gammaproteobacteria (51.1%), 32 in Firmicutes (35.6%), 10 in Betaproteobacteria (11.1%), and 2 in Alphaproteobacteria (2.2%). The dominant bacteria species were Pseudomonas and Bacillus.

CONCLUSION:

There was abundant species diversity of bacteria isolated from cysts Heterodera glycines in Heilongjiang, and these bacteria may play a physical and ecological roles in nematodes.

PMID:
23115975
[PubMed - indexed for MEDLINE]
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