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Toxicol Ind Health. 2014 Nov;30(10):888-95. doi: 10.1177/0748233712466134. Epub 2012 Oct 31.

Protective effects of Ankaferd blood stopper on aspirin-induced oxidative mucosal damage in a rat model of gastric injury.

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  • 1Department of Biochemistry, Faculty of Medicine, Fatih University, Ankara, Turkey.
  • 2Department of Biochemistry, Numune Training and Research Hospital, Ankara, Turkey.
  • 3Department of Pathology, Faculty of Medicine, Fatih University, Ankara, Turkey.
  • 4Department of Histology, Faculty of Medicine, Fatih University, Ankara, Turkey.
  • 5Department of Pharmacology, Faculty of Medicine, Fatih University, Ankara, Turkey.
  • 6Department of Biochemistry, Faculty of Medicine, Fatih University, Ankara, Turkey


The exposure of gastric mucosa to damaging factors, such as ethanol and some therapeutic drugs, produces pathological changes: inflammatory process, hemorrhagic erosions and even acute ulcers. Ankaferd blood stopper (ABS) comprises a standardized mixture of five different plant extracts. The purpose of our present investigations is to explain the participation of reactive oxygen species in acute gastric mucosal damage by acetylsalicylic acid (ASA) and the effects of new hemostatic agent ABS. Experiments were carried out on 23 male Wistar rats. To assess gastric mucosal damage, biochemical and histopathological data were used. The colorimetric assays were used to determine the malondialdehyde (MDA) and superoxide dismutase (SOD) activity. The level of myeloperoxidase (MPO) activity, the level of nitric oxide (NO) and the proinflammatory cytokine tumor necrosis factor-α (TNF-α) were measured by enzyme-linked immunosorbent assay technique. We demonstrated that the biological effects of ROS were estimated by measuring the tissue and plasma levels of MDA, the products of lipid peroxidation, as well as the activity of SOD and the scavenger of ROS produced by ASA in the experiment group. Moreover, it was found that MPO activity as well as NO and TNF-α levels also demonstrated significant improvement by ABS treatment. The pathogenesis of experimental ASA-induced mucosal damage in rat stomach includes the generation of ROS that seems to play an important role, due to the generation of lipid peroxides, accompanied by the impairment of antioxidative enzyme activity of cells. ABS appeared to attenuate the oxidative and inflammatory changes caused by ASA-induced gastric mucosal damage in rats.

© The Author(s) 2012.


Acetylsalicylic acid; Ankaferd; gastric mucosal damage; inflammation; oxidative stress

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