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Am J Bot. 2012 Nov;99(11):e431-3. doi: 10.3732/ajb.1200165. Epub 2012 Oct 29.

Isolation and characterization of microsatellite markers for an important tropical tree, Aquilaria malaccensis (Thymelaeaceae).

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  • 1Genetic Laboratory, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.

Abstract

PREMISE OF THE STUDY:

Aggressive collections and trade activities in recent decades have resulted in heavy pressure on the natural stands of Aquilaria malaccensis and concerns over its long-term survival potential. To aid DNA profiling and assessment of its genetic diversity, microsatellite markers were developed for the species.

METHODS AND RESULTS:

Seventeen polymorphic microsatellite markers were developed for A. malaccensis using an enrichment protocol. The markers were screened on 24 samples from a natural population. The number of alleles ranged from two to 11, and the observed heterozygosity ranged from 0.042 to 0.957. No significant deviation from Hardy-Weinberg equilibrium was detected after conservative Bonferroni correction.

CONCLUSIONS:

This is the first report on the development of microsatellite markers in A. malaccensis. The markers will be used to establish a DNA profiling database and to estimate the genetic diversity and population genetic structure of the species.

PMID:
23108468
[PubMed - indexed for MEDLINE]
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