Display Settings:

Format

Send to:

Choose Destination
Methods Mol Biol. 2013;940:77-86. doi: 10.1007/978-1-62703-110-3_7.

Biolistic transformation of Caenorhabditis elegans.

Author information

  • 1Hubrecht Institute and University Medical Center Utrecht, Utrecht, The Netherlands.

Abstract

The ability to generate transgenic animals to study gene expression and function is a powerful and important part of the Caenorhabditis elegans genetic toolbox. Transgenic animals can be created by introducing exogenous DNA into the worm germline either by microinjection or by microparticle bombardment (biolistic transformation). In this chapter we describe a simple and robust protocol to generate transgenic C. elegans animals by biolistic transformation with gold particles using the Bio-Rad PDS-1000/He system with Hepta adapter and unc-119 selection marker. We also point out the steps that need special attention to achieve successful transformations.

PMID:
23104335
[PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Springer
    Loading ...
    Write to the Help Desk