Format

Send to:

Choose Destination
See comment in PubMed Commons below
ScientificWorldJournal. 2012;2012:205038. doi: 10.1100/2012/205038. Epub 2012 Oct 11.

Genomic profiling of a human leukemic monocytic cell-line (THP-1) exposed to alpha particle radiation.

Author information

  • 1Consumer and Clinical Radiation Protection Bureau, Health Canada, Healthy Environment and Consumer Safety Branch, Ottawa, ON, Canada K1A 0K9. vinita chauhan@hc-sc.gc.ca

Abstract

This study examined alpha (α-) particle radiation effects on global changes in gene expression in human leukemic monocytic cells (THP-1) for the purposes of mining for candidate biomarkers that could be used for the development of a biological assessment tool. THP-1 cells were exposed to α-particle radiation at a dose range of 0 to 1.5 Gy. Twenty-four hours and three days after exposure gene expression was monitored using microarray technology. A total of 16 genes were dose responsive and classified as early onset due to their expression 24 h after exposure. Forty-eight transcripts were dose responsive and classified as late-onset as they were expressed 72 h after exposure. Among these genes, 6 genes were time and dose responsive and validated further using alternate technology. These transcripts were upregulated and associated with biological processes related to immune function, organelle stability and cell signalling/communication. This panel of genes merits further validation to determine if they are strong candidate biomarkers indicative of α-particle exposure.

PMID:
23097634
[PubMed - indexed for MEDLINE]
PMCID:
PMC3477555
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Hindawi Publishing Corporation Icon for PubMed Central
    Loading ...
    Write to the Help Desk