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Arch Med Res. 2012 Oct;43(7):555-62. doi: 10.1016/j.arcmed.2012.09.010. Epub 2012 Oct 17.

Diagnostic performances of hepatitis C virus-NS4 antigen in patients with different liver pathologies.

Author information

  • 1Biotechnology Research Center, New Damietta City, Egypt. amattallah@hotmail.com

Abstract

BACKGROUND AND AIMS:

Hepatitis C virus (HCV) has emerged as the major pathogen of liver disease worldwide. The aim of this study was to quantitate and evaluate the performance of HCV-NS4 antigen as an alternative approach for confirmation of viremia.

METHODS:

Detection of HCV-NS4 was assessed in 883 patients with chronic hepatitis C. Areas under the ROC curves (AUC) were used to assess and compare diagnostic accuracy of ELISA for HCV-NS4 with quantitative HCV-RNA as a gold standard.

RESULTS:

HCV-NS4 was identified at 27 kDa using Western blot. AUC for HCV-NS4 detection was 0.95 for all patients with different liver pathologies: 0.93 for liver fibrosis (LF), 0.95 for liver cirrhosis (LC) and 0.98 for hepatocellular carcinoma (HCC). The mean ± SD (μg/mL) of HCV-NS4 in LF was 94.2 ± 55.6; in LC was 99.3 ± 64.8 and in HCC was 124.9 ± 70.3.

CONCLUSIONS:

HCV-NS4 antigen detection using ELISA is a reliable test in the confirmation of HCV infection.

Copyright © 2012 IMSS. Published by Elsevier Inc. All rights reserved.

PMID:
23085447
[PubMed - indexed for MEDLINE]
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