High-resolution homonuclear 2D NMR of carbon-13 enriched metabolites and their mixtures

J Magn Reson. 2012 Dec:225:10-3. doi: 10.1016/j.jmr.2012.09.006. Epub 2012 Sep 24.

Abstract

High-resolution 2D (13)C-(13)C NMR correlation spectra of uniformly (13)C-labeled molecules in solution are obtained by homonuclear (13)C-decoupling along both dimensions by the application of indirect covariance NMR to constant-time NMR spectra. The spectra are optimally suited for chemical structure elucidation and molecular identification of the components of complex mixtures, such as ones from uniformly (13)C-labeled cell cultures.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Algorithms
  • Amino Acids / analysis
  • Carbon Isotopes
  • Glucose / analysis
  • Glucose / chemistry
  • Isotope Labeling
  • Nuclear Magnetic Resonance, Biomolecular / methods*

Substances

  • Amino Acids
  • Carbon Isotopes
  • Glucose