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Cell Rep. 2012 Oct 25;2(4):991-1001. doi: 10.1016/j.celrep.2012.09.011. Epub 2012 Oct 11.

A GAL4-driver line resource for Drosophila neurobiology.

Author information

  • 1Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.

Abstract

We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.

Copyright © 2012 The Authors. Published by Elsevier Inc. All rights reserved.

PMID:
23063364
[PubMed - indexed for MEDLINE]
PMCID:
PMC3515021
Free PMC Article

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