Single-exposure complementary aperture phase microscopy with polarization encoding

Opt Lett. 2012 Sep 15;37(18):3798-800. doi: 10.1364/ol.37.003798.

Abstract

We introduce a single-exposure widefield system of producing phase gradient images by dividing the illumination and detection apertures into two oblique complementary components and encoding them using polarization. A Wollaston prism splits the images formed by the two respective aperture halves to allow both components to be simultaneously imaged by a single camera. By producing images characteristically similar to differential interference contrast while using a darkfield illumination scheme, sensitivity to weak phase gradients is improved.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Equipment Design
  • Equipment Failure Analysis
  • Image Enhancement / instrumentation*
  • Lenses*
  • Microscopy, Phase-Contrast / instrumentation*
  • Refractometry / instrumentation*