Format

Send to:

Choose Destination
See comment in PubMed Commons below
Fertil Steril. 2013 Jan;99(1):118-24. doi: 10.1016/j.fertnstert.2012.09.004. Epub 2012 Oct 3.

Involvement of the inflammasome in abnormal semen quality of men with spinal cord injury.

Author information

  • 1Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, Florida 33136, USA.

Abstract

OBJECTIVE:

To study the mechanism leading to elevated semen cytokines in men with spinal cord injury (SCI) and to understand if inflammasome pathways are involved in this process. To investigate inflammasome components and end-product cytokines in semen of SCI and control subjects.

DESIGN:

Prospective study.

SETTING:

Major university medical center.

PATIENT(S):

Men with and without SCI (n = 28 per group).

INTERVENTION(S):

None.

MAIN OUTCOME MEASURE(S):

Seminal plasma concentrations of caspase-1, interleukin (IL) 1β, and IL-18 were quantified by ELISA. Caspase-1 in sperm fractions and apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC) in seminal plasma and sperm fractions were identified by Western blot. Localization of proteins in sperm was accomplished by immunocytochemistry.

RESULT(S):

ASC, caspase-1, IL-1β, and IL-18 concentrations were elevated in the seminal plasma of SCI subjects compared with control subjects. ASC and caspase-1 were elevated in sperm cells of SCI subjects. Immunocytochemistry revealed that ASC was located in the acrosome, equatorial segment, and midpiece, and caspase-1 in the midpiece.

CONCLUSION(S):

This study provides the first evidence of ASC in human semen and demonstrates the involvement of inflammasome proteins in semen of men with SCI. These findings suggest an immunologic basis for abnormal semen quality in men with SCI.

Copyright © 2013 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

PMID:
23040525
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk