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Genome Biol. 2012 Oct 3;13(10):R86. doi: 10.1186/gb-2012-13-10-r86.

Enhanced top-down characterization of histone post-translational modifications.

Abstract

Post-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. We report a novel online two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) platform for high-throughput and sensitive characterization of histone PTMs at the intact protein level. The platform enables unambiguous identification of 708 histone isoforms from a single 2D LC-MS/MS analysis of 7.5 µg purified core histones. The throughput and sensitivity of comprehensive histone modification characterization is dramatically improved compared with more traditional platforms.

PMID:
23034525
[PubMed - in process]
PMCID:
PMC3491414
Free PMC Article

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