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Int J Nanomedicine. 2012;7:4961-72. doi: 10.2147/IJN.S32069. Epub 2012 Sep 14.

Amphiphilic graft copolymer based on poly(styrene-co-maleic anhydride) with low molecular weight polyethylenimine for efficient gene delivery.

Author information

  • 1School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.

Abstract

BACKGROUND AND METHODS:

A new amphiphilic comb-shaped copolymer (SP) was synthesized by conjugating poly(styrene-co-maleic anhydride) with low molecular weight polyethyleneimine for gene delivery. Fourier transform infrared spectrum, (1)H nuclear magnetic resonance, and gel permeation chromatography were used to characterize the graft copolymer.

RESULTS:

The buffering capability of SP was similar to that of polyethyleneimine within the endosomal pH range. The copolymer could condense DNA effectively to form complexes with a positive charge (13-30 mV) and a small particle size (130-200 nm) at N/P ratios between 5 and 20, and protect DNA from degradation by DNase I. In addition, SP showed much lower cytotoxicity than polyethyleneimine 25,000. Importantly, the gene transfection activity and cellular uptake of SP-DNA complexes were all markedly higher than that of complexes of polyethyleneimine 25,000 and DNA in MCF-7 and MCF-7/ADR cell lines.

CONCLUSION:

This work highlights the promise of SP as a safe and efficient synthetic vector for DNA delivery.

KEYWORDS:

DNA; gene delivery; poly(styrene-co-maleic anhydride); polyethylenimine

PMID:
23028224
[PubMed - indexed for MEDLINE]
PMCID:
PMC3446863
Free PMC Article

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