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Int Arch Allergy Immunol. 2013;160(2):152-60. doi: 10.1159/000339857. Epub 2012 Sep 25.

Cytokine secretion from human monocytes potentiated by P-selectin-mediated cell adhesion.

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  • 1Department of Microbiology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan.

Abstract

BACKGROUND/AIM:

P-selectin is a carbohydrate-recognizing cell adhesion molecule expressed on activated platelets and endothelial cells. It plays a crucial role in the recruitment of leukocytes to inflammatory and hemorrhagic sites. Cell adhesion mediated by P-selectin induces leukocyte activation, such as the generation of reactive oxygen species and the expression of blood coagulation factors. We assessed how P-selectin-mediated cell adhesion affects cytokine secretion from monocytes.

METHODS:

Human peripheral blood monocytes were cultured in a plate that had been coated with P-selectin purified from human platelets, and cytokines released in the culture supernatant from monocytes were determined by ELISA.

RESULTS:

The secretion of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8, IL-12 and macrophage inflammatory protein-1β increased 3- to 10-fold in response to P-selectin compared with unstimulated monocytes. We next examined the effects of cytokine treatment of monocytes on their susceptibility to P-selectin. The secretion of TNF-α from monocytes in response to P-selectin was increased when monocytes were preincubated with granulocyte/macrophage colony-stimulating factor, monocyte chemotactic protein-1 or interferon-γ (IFN-γ); IFN-γ was the most effective in potentiating TNF-α secretion from monocytes.

CONCLUSION:

These results suggest that the interaction of monocytes with P-selectin plays an important role not only in their trafficking but also in the regulation of cytokine production by these cells.

Copyright © 2012 S. Karger AG, Basel.

PMID:
23018521
[PubMed - indexed for MEDLINE]
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