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Biosens Bioelectron. 2013 Mar 15;41:163-7. doi: 10.1016/j.bios.2012.08.018. Epub 2012 Aug 30.

Highly sensitive detection of organophosphorus pesticides by acetylcholinesterase-coated thin film bulk acoustic resonator mass-loading sensor.

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  • 1Qingdao Key Laboratory of Terahertz Technology, Department of Physics, School of Science, Shandong University of Science and Technology, Qianwangang Road 579 #, Qingdao 266510, PR China. phychenda@163.com

Abstract

An acetylcholinesterase-coated thin film bulk acoustic resonator has been developed for the detection of organophosphorus pesticides. The thin film bulk acoustic resonator acts as a robust mass-sensitive transducer for bio-sensing. This device works in thickness shear mode with a resonance at 1.97 GHz. The detection is based on the inhibitory effects of organophosphorus compounds on the enzymatic activity of the acetylcholinesterase immobilized on one of the faces of the acoustic resonator. The enzyme reaction in the substrate solution and the inhibitory effect is observed are real time by measuring the frequency shift. The presence of organophosphorus pesticides can be detected from the diminution of the frequency shift compared with the levels found in their absence. The device exhibits linear responses, good reproducibility, simple operation, portability and a low detection limit of 5.3×10(-11) M for paraoxon. The detection results of organophosphorus pesticide residues in practical samples show that the proposed sensor has the feasibility and sensing accuracy comparable to gas chromatography.

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID:
23017678
[PubMed - indexed for MEDLINE]
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