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Analyst. 2012 Dec 7;137(23):5457-82. doi: 10.1039/c2an35697j. Epub 2012 Sep 26.

Applications of reversible covalent chemistry in analytical sample preparation.

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  • 1BAM Federal Institute for Materials Research and Testing, Richard-Willst├Ątter-Str 11, 12489 Berlin, Germany. ds@dsscience.de

Abstract

Reversible covalent chemistry (RCC) adds another dimension to commonly used sample preparation techniques like solid-phase extraction (SPE), solid-phase microextraction (SPME), molecular imprinted polymers (MIPs) or immuno-affinity cleanup (IAC): chemical selectivity. By selecting analytes according to their covalent reactivity, sample complexity can be reduced significantly, resulting in enhanced analytical performance for low-abundance target analytes. This review gives a comprehensive overview of the applications of RCC in analytical sample preparation. The major reactions covered include reversible boronic ester formation, thiol-disulfide exchange and reversible hydrazone formation, targeting analyte groups like diols (sugars, glycoproteins and glycopeptides, catechols), thiols (cysteinyl-proteins and cysteinyl-peptides) and carbonyls (carbonylated proteins, mycotoxins). Their applications range from low abundance proteomics to reversible protein/peptide labelling to antibody chromatography to quantitative and qualitative food analysis. In discussing the potential of RCC, a special focus is on the conditions and restrictions of the utilized reaction chemistry.

PMID:
23013801
[PubMed - indexed for MEDLINE]
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