Application of different chemometric strategies to voltammetric and UV-vis spectroscopic data to obtain a complexation model: study of the Cu(II) binding with the phytohormone 6-benzylaminopurine

Analyst. 2012 Nov 21;137(22):5420-7. doi: 10.1039/c2an35600g.

Abstract

The complexation processes of Cu(II) by the phytohormone and the possible antitumoral agent 6-benzylaminopurine (BAP) were studied by Differential Pulse Polarography (DPP) and ultraviolet-visible spectroscopy (UV-vis) in combination with chemometric programs such as Multivariate Curve Resolution by Alternating Least-Squares (MCR-ALS) and Gaussian Peak Adjustment (GPA). All data confirm the formation of the predominant 1 : 1 and 1 : 2 Cu(2+) : BAP complexes. The corroboration of the presented stoichiometries was performed by ElectroSpray Ionization-Mass Spectrometric (ESI-MS) experiments of the Cu(II)-BAP system at different ratios.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry*
  • Benzyl Compounds / chemistry*
  • Copper / chemistry*
  • Electrochemical Techniques
  • Ions / chemistry
  • Least-Squares Analysis
  • Models, Chemical*
  • Purines / chemistry*
  • Spectrometry, Mass, Electrospray Ionization
  • Spectrophotometry, Ultraviolet*

Substances

  • Antineoplastic Agents
  • Benzyl Compounds
  • Ions
  • Purines
  • Copper
  • benzylaminopurine