Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
RNA. 2012 Nov;18(11):2012-9. doi: 10.1261/rna.035501.112. Epub 2012 Sep 24.

TRBP alters human precursor microRNA processing in vitro.

Author information

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA.

Abstract

MicroRNAs play central roles in controlling gene expression in human cells. Sequencing data show that many miRNAs are produced at different levels and as multiple isoforms that can vary in length at their 5' or 3' ends, but the biogenesis and functional significance of these RNAs are largely unknown. We show here that the human trans-activation response (TAR) RNA binding protein (TRBP), a known molecular partner of the miRNA processing enzyme Dicer, changes the rates of pre-miRNA cleavage in an RNA-structure-specific manner. Furthermore, TRBP can trigger the generation of iso-miRNAs (isomiRs) that are longer than the canonical sequence by one nucleotide. We show that this change in miRNA processing site can alter guide strand selection, resulting in preferential silencing of a different mRNA target. These results implicate TRBP as a key regulator of miRNA processing and targeting in humans.

PMID:
23006623
[PubMed - indexed for MEDLINE]
PMCID:
PMC3479391
Free PMC Article

Images from this publication.See all images (4)Free text

FIGURE 1.
FIGURE 2.
FIGURE 3.
FIGURE 4.
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk