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Genome. 2012 Sep;55(9):691-5. doi: 10.1139/G2012-058. Epub 2012 Sep 24.

Genotyping SSR length variants by isothermal DNA amplification.

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  • 1John Bingham Laboratory, National Institute of Agricultural Botany, Cambridge CB3 0LE, UK. david.lee@niab.com

Abstract

Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be consistent with analysis performed by PCR. Furthermore, we test the sensitivity of the assay and show it to amplify from near single copy target.

[PubMed - indexed for MEDLINE]
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