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Zhongguo Zhong Yao Za Zhi. 2012 Jun;37(11):1607-9.

[Quantitative determination of sibricose A5 and sibricose A6 in Polygalae radix].

[Article in Chinese]

Author information

  • 1School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. sunchangqingscq@126.com

Abstract

OBJECTIVE:

To establish the method for quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix by HPLC.

METHOD:

The ultrasonic extracting method was applied in sample pre-treatment. The HPLC procedure was performed on the chromatographic column of Agela Promosil C18 (4.6 mm x 250 mm, 5 microm), the mobile phase was acetonitrile-0.1% phosphoric acid water solution (10:90). The detection wavelength was 330 nm and flow velocity was 1 mL x min(-1). The column temperature was 30 degrees C.

RESULT:

The method has good linearity in the ranges of 0.0087-0.0694 g x L(-1) (r = 0.9993) for sibricose A5, 0.0090-0.0723 g x L(-1) (r=0.9991) for sibricose A6. The average recoveries of sibricose A5 and sibricose A6 were 101.7%, 97.87%, with the RSD of 1.7%, 1.6%, respectively.

CONCLUSION:

The method was simple, quick accurate and reliable. It is appropriate for the quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix.

PMID:
22993991
[PubMed - indexed for MEDLINE]
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