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J Neurooncol. 2012 Dec;110(3):305-13. doi: 10.1007/s11060-012-0973-6. Epub 2012 Sep 17.

Characterization of a diffuse intrinsic pontine glioma cell line: implications for future investigations and treatment.

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  • 1Department of Neurological Surgery, Brain Tumor Research Center, University of California San Francisco, San Francisco, CA 94143-0520, USA.

Abstract

Diffuse intrinsic pontine gliomas arise almost exclusively in children, and despite advances in treatment, the majority of patients die within 2 years after initial diagnosis. Because of their infiltrative nature and anatomic location in an eloquent area of the brain, most pontine gliomas are treated without a surgical biopsy. The corresponding lack of tissue samples has resulted in a limited understanding of the underlying genetic and molecular biologic abnormalities associated with pontine gliomas, and is a substantial obstacle for the preclinical testing of targeted therapeutic agents for these tumors. We have established a human glioma cell line that originated from surgical biopsy performed on a patient with a pontine glioma. To insure sustainable in vitro propagation, tumor cells were modified with hTERT (human telomerase ribonucleoprotein reverse transcriptase), and with a luciferase reporter to enable non-invasive bioluminescence imaging. The hTERT modified cells are tumorigenic in athymic rodents, and produce brainstem tumors that recapitulate the infiltrative growth of brainstem gliomas in patients.

PMID:
22983601
[PubMed - indexed for MEDLINE]
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