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Clin Biochem. 2012 Dec;45(18):1689-93. doi: 10.1016/j.clinbiochem.2012.09.001. Epub 2012 Sep 12.

Development of an Au/ZnO thin film surface plasmon resonance-based biosensor immunoassay for the detection of carbohydrate antigen 15-3 in human saliva.

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  • 1Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.



To evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva.


We measured the presence of the tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva using 2 different surface plasmon resonance (SPR) systems. To compare the sensitivity of an SPR biosensor based on thin-film Au/ZnO and the Biacore SPR system, we prepared CA15-3 samples in saliva and analyzed intensity responses to the samples at various concentrations of CA15-3.


The linear detection range of CA15-3 in human saliva with the SPR system based on thin-film Au/ZnO was 2.5-20 U/mL (the cut-off point in cancer patients is around 4 U/mL). The linear range with the Biacore SPR system was 40-300 U/mL.


These results show that thin-film Au/ZnO-based SPR systems have higher sensitivity and can be used for measuring the levels of CA15-3 in human saliva without concentrating the samples.

Copyright © 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

[PubMed - indexed for MEDLINE]
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