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Front Microbiol. 2012 Sep 4;3:319. doi: 10.3389/fmicb.2012.00319. eCollection 2012.

Identifying viral parameters from in vitro cell cultures.

Author information

  • 1Faculty of Sciences, Department of Biology, Kyushu University Higashi-ku, Fukuoka, Japan.

Abstract

Current in vitro cell culture studies of viral replication deliver detailed time courses of several virological variables, like the amount of virions and the number of target cells, measured over several days of the experiment. Each of these time points solely provides a snap-shot of the virus infection kinetics and is brought about by the complex interplay of target cell infection, and viral production and cell death. It remains a challenge to interpret these data quantitatively and to reveal the kinetics of these underlying processes to understand how the viral infection depends on these kinetic properties. In order to decompose the kinetics of virus infection, we introduce a method to "quantitatively" describe the virus infection in in vitro cell cultures, and discuss the potential of the mathematical based analyses for experimental virology.

KEYWORDS:

in vitro experiment; mathematical modeling; quantification; virus infection

PMID:
22969758
[PubMed]
PMCID:
PMC3432869
Free PMC Article

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