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J Neurosci Methods. 2012 Sep 30;210(2):272-80. doi: 10.1016/j.jneumeth.2012.07.024. Epub 2012 Aug 9.

Evaluation of transcranial Doppler flow velocity changes in intracerebral hemorrhage rats using ultrasonography.

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  • 1Interdisciplinary Division of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region.


This study investigated the blood flow velocity changes in seven major arteries in rat brain before and after intracerebral hemorrhage (ICH) using high frequency transcranial Doppler (TCD) ultrasonography (13-4 MHz). Eighteen adult Sprague Dawley rats received either the collagenase-injection surgery (ICH, n=12) or the saline-injection surgery (control, n=6) after baseline TCD flow velocity values were recorded. The TCD flow velocity changes were measured at 0.5h after the surgery and daily for the following 8 days in seven major cerebral arteries, including bilateral internal carotid arteries, bilateral middle cerebral arteries (MCAs), bilateral posterior cerebral arteries (PCAs), and basilar artery. The results showed a significant decrease of TCD flow velocity in the right MCA at 0.5 h and 24 h after the collagenase-injection surgery, and in the right PCA at 0.5 h in the ICH group rats. The TCD flow velocities in these two arteries gradually increased and then returned to the baseline values in the following days. The control group rats did not show significant changes in TCD flow velocity in all monitored arteries. This study demonstrates the feasibility and reliability of monitoring TCD flow velocity in cerebral arteries using ultrasonography technique in a rat ICH model. The results of this study extend our knowledge in the cerebrovascular changes during intracranial hemorrhage and suggest a possibility of clinical application of TCD ultrasonography in studying the dynamic cerebral circulation after strokes. Moreover, this method could be extensively applied in further studies using potential neuroprotective treatments that affect the cerebral dynamics in the intracerebral hemorrhage.

Copyright © 2012 Elsevier B.V. All rights reserved.

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