Mapping the mechanism of the resorcinol ring formation catalyzed by ArsB, a type III polyketide synthase from Azotobacter vinelandii

Chembiochem. 2012 Oct 15;13(15):2212-7. doi: 10.1002/cbic.201200346. Epub 2012 Sep 7.

Abstract

Who's first? Aldol cyclization occurs before hydrolysis in the resorcinol ring formation catalyzed by the type III polyketide synthase, ArsB. Synthetic C(20)-TKA was not converted to alkylresorcinol by ArsB, but rather inhibited the enzyme activity, thus indicating that C(20)-TKA is not an intermediate in ArsB-catalyzed alkylresorcinol formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azotobacter vinelandii / chemistry
  • Azotobacter vinelandii / enzymology*
  • Azotobacter vinelandii / metabolism
  • Cyclization
  • Hydrolysis
  • Polyketide Synthases / metabolism*
  • Resorcinols / chemistry
  • Resorcinols / metabolism*
  • Substrate Specificity

Substances

  • Resorcinols
  • Polyketide Synthases
  • resorcinol