Send to

Choose Destination
See comment in PubMed Commons below
Top Curr Chem. 2013;331:165-92. doi: 10.1007/128_2012_364.

Chemical and biochemical applications of MALDI TOF-MS based on analyzing the small organic compounds.

Author information

  • 1Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Science, LingLing Road 345, 200032, Shanghai, People's Republic of China.


This review focuses on the recent applications of matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF-MS) technologies for analysis of small molecular compounds (SMCs) on qualitative and quantitative levels to provide valuable information for the relative chemical and biochemical researches. We summarized the approaches to minimize the interference in low m/z range and ion suppression effects by adding chemical reagents, sample cleaning and preparation, matrix selection and performing chromatographic separation before MALDI TOF-MS analysis. Meanwhile, we discussed the strategies to enhance the MALDI TOF-MS detection sensitivity and selectivity by derivatization to attach the "charge tags" to SMCs. In addition, the mass spectrometric imaging (MSI) methods for locating the target SMCs in bio-tissues by MALDI TOF-MS were reviewed. Furthermore, the applications of MALDI TOF-MS for monitoring enzyme reactions and the screening of their inhibitors were also presented. Finally, the chemical applications of MALDI TOF-MS on characterization of small molecular transition-metal complexes and monitoring the organic reactions, especially the polymerization reactions, were discussed.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Write to the Help Desk