Total and ultrafiltered calcium, total and inorganic phosphorus in blood serum and diurnal secretion of calcium and phosphorus with urine were determined before (baseline data collection) and on days 30, 60 and 105 of human subjects' isolation. Calcium and phosphorus content in hair was determined during BDC and on day 105 of the experiment. The measurements were fulfilled with the help of inductively coupled plasma atomic emission spectrometry. Levels of total and ultrafiltered calcium, total and inorganic phosphorus as well as urine calcium and phosphorus in daily urine and hair varied within admissible physiological ranges unfailingly at all the times of investigation. Shifts in the serum calcium and phosphorus balance occur at the expense of their physiologically active forms representing an immediate reaction to the experimental conditions. Calcium and phosphorus levels deviated from baseline values after 30 days of isolation and demonstrated stability till day 105.