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J Biol Eng. 2012 Sep 4;6(1):14. doi: 10.1186/1754-1611-6-14.

The Constructor: a web application optimizing cloning strategies based on modules from the registry of standard biological parts.

Author information

  • 1iGEM Wageningen UR, Wageningen University, Wageningen, The Netherlands. Mark.vanPassel@wur.nl.

Abstract

Synthetic biology is an emerging field that combines molecular biology with engineering principles, which requires abstraction levels applied to a modular biological componentry. The Registry of Standard Biological Parts harbours such a repository of standardized parts, and thereby facilitates the combination of complex molecular modules to novel genetic circuits and devices. However, since finding the best parts for a pre-determined genetic design can be time consuming, we devised the Constructor, a web tool that recommends the smallest number of cloning steps for pre-designed circuits, and implements user-defined quality checks.We present the Constructor ( http://www.systemsbiology.nl/the_constructor) as a constructive web tool that simplifies the in silico assembly of pre-designed gene circuitries from standard parts, reducing both planning and subsequent cloning time.

PMID:
22947262
[PubMed]
PMCID:
PMC3520746
Free PMC Article

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