[The protective effect of chlorophyllin against oxidative damage and its mechanism]

Zhonghua Nei Ke Za Zhi. 2012 Jun;51(6):466-70.
[Article in Chinese]

Abstract

Objective: To investigate whether chlorophyllin could protect human umbilical vein endothelial cell (HUVEC) against oxidative damage by inducing the expression of heme oxygenase-1 (HO-1) and to explore the underlying mechanism.

Methods: The cellular protection of chlorophyllin against oxidative damage was detected by cell-survival assay with flow cytometry. The level of free radicals was detected directly by electron spin resonance spectra. The induced expression of HO-1 was shown by RT-PCR, Western blot, immunofluorescence confocal laser microscopy and enzymatic activity test. Whether the activation of PI3K/Akt pathway was involved was detected by Western blot.

Results: Chlorophyllin could protect HUVEC against oxidative damage caused by H2O2 via scavenging the excessive free radicals. Chlorophyllin treatment could induce expression of HO-1 in a dose- and time-dependent manner. The activation of PI3K/Akt pathway was required in the induction of HO-1. LY294002, the specific inhibitor of PI3K, could suppress the activation of PI3K/Akt and the induced expression of HO-1 in a dose-dependent manner.

Conclusions: Chlorophyllin shows cellular protection against oxidative damage by counteracting the excessive free radicals. Up-regulation of HO-1 expression plays a pivotal role in the protection of chlorophyllin, while the activation of PI3K/Akt signaling pathway is required in the induction of HO-1.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cells, Cultured
  • Chlorophyllides / pharmacology*
  • Chromones / pharmacology
  • Free Radical Scavengers / pharmacology*
  • Heme Oxygenase-1
  • Human Umbilical Vein Endothelial Cells / drug effects
  • Humans
  • Morpholines / pharmacology
  • Oxidative Stress / drug effects*
  • Phosphoinositide-3 Kinase Inhibitors
  • Proto-Oncogene Proteins c-akt / metabolism
  • Reactive Oxygen Species / metabolism*
  • Signal Transduction

Substances

  • Chlorophyllides
  • Chromones
  • Free Radical Scavengers
  • Morpholines
  • Phosphoinositide-3 Kinase Inhibitors
  • Reactive Oxygen Species
  • 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
  • HMOX1 protein, human
  • Heme Oxygenase-1
  • Proto-Oncogene Proteins c-akt
  • chlorophyllin