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Methods Mol Biol. 2012;920:221-43. doi: 10.1007/978-1-61779-998-3_16.

Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts.

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  • 1Hubrecht Institute, KNAW and University Medical Center Utrecht, Utrecht, The Netherlands.


Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.

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