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Am J Bot. 2012 Sep;99(9):e369-71. doi: 10.3732/ajb.1200077. Epub 2012 Aug 30.

Development of chromosome-arm-specific microsatellite markers in Triticum aestivum (Poaceae) using NGS technology.

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  • 1State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.

Abstract

PREMISE OF THE STUDY:

The aim of this study was to assess the feasibility of developing chromosome-arm-specific microsatellite markers in wheat on a large scale based on chromosome survey sequences obtained with next-generation sequencing (NGS) technology.

METHODS AND RESULTS:

The Illumina Hi Seq2000 sequencing platform was used to sequence DNA of isolated wheat chromosome-arm 7DL. The data were assembled and microsatellite loci were identified computationally. In total, 16315 microsatellites were identified from 161061 assembled contigs. Thirty-three markers were randomly selected for validation across 20 diverse wheat cultivars. Two nulli-tetrasomic stocks were also screened to validate the specificity of the newly developed markers.

CONCLUSIONS:

This is the first study on identification of chromosome-arm-specific microsatellite markers using NGS technology. These new chromosome-arm-specific markers will facilitate saturation of the 7DL genetic map, and their availability will support genetic mapping and positional cloning in wheat.

PMID:
22935363
[PubMed - indexed for MEDLINE]
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