Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Malar J. 2012 Aug 28;11:302. doi: 10.1186/1475-2875-11-302.

High-throughput sorting of mosquito larvae for laboratory studies and for future vector control interventions.

Author information

  • 1Institut de Biologie MolĂ©culaire et Cellulaire, INSERM U963, CNRS UPR9022, 15 rue RenĂ© Descartes, 67084 Strasbourg, France.

Abstract

BACKGROUND:

Mosquito transgenesis offers new promises for the genetic control of vector-borne infectious diseases such as malaria and dengue fever. Genetic control strategies require the release of large number of male mosquitoes into field populations, whether they are based on the use of sterile males (sterile insect technique, SIT) or on introducing genetic traits conferring refractoriness to disease transmission (population replacement). However, the current absence of high-throughput techniques for sorting different mosquito populations impairs the application of these control measures.

METHODS:

A method was developed to generate large mosquito populations of the desired sex and genotype. This method combines flow cytometry and the use of Anopheles gambiae transgenic lines that differentially express fluorescent markers in males and females.

RESULTS:

Fluorescence-assisted sorting allowed single-step isolation of homozygous transgenic mosquitoes from a mixed population. This method was also used to select wild-type males only with high efficiency and accuracy, a highly desirable tool for genetic control strategies where the release of transgenic individuals may be problematic. Importantly, sorted males showed normal mating ability compared to their unsorted brothers.

CONCLUSIONS:

The developed method will greatly facilitate both laboratory studies of mosquito vectorial capacity requiring high-throughput approaches and future field interventions in the fight against infectious disease vectors.

PMID:
22929810
[PubMed - indexed for MEDLINE]
PMCID:
PMC3470999
Free PMC Article

Images from this publication.See all images (4)Free text

Figure 1
Figure 2
Figure 3
Figure 4
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for BioMed Central Icon for PubMed Central
    Loading ...
    Write to the Help Desk