Format

Send to:

Choose Destination
See comment in PubMed Commons below
PLoS One. 2012;7(8):e42133. doi: 10.1371/journal.pone.0042133. Epub 2012 Aug 22.

In vivo optical imaging of tumor and microvascular response to ionizing radiation.

Author information

  • 1Ontario Cancer Institute, Princess Margaret Hospital, University Health Network, Toronto, Ontario, Canada.

Abstract

Radiotherapy is a widely used cancer treatment. However, understanding how ionizing radiation affects tumor cells and their vasculature, particularly at cellular, subcellular, genetic, and protein levels, has been limited by an inability to visualize the response of these interdependent components within solid tumors over time and in vivo. Here we describe a new preclinical experimental platform combining intravital multimodal optical microscopy for cellular-level longitudinal imaging, a small animal x-ray microirradiator for reproducible spatially-localized millimeter-scale irradiations, and laser-capture microdissection of ex vivo tissues for transcriptomic profiling. Using this platform, we have developed new methods that exploit the power of optically-enabled microscopic imaging techniques to reveal the important role of the tumor microvasculature in radiation response of tumors. Furthermore, we demonstrate the potential of this preclinical platform to study quantitatively--with cellular and sub-cellular details--the spatio-temporal dynamics of the biological response of solid tumors to ionizing radiation in vivo.

PMID:
22927920
[PubMed - indexed for MEDLINE]
PMCID:
PMC3425534
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk