Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nat Struct Mol Biol. 2012 Sep;19(9):884-92. doi: 10.1038/nsmb.2312. Epub 2012 Aug 26.

Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange.

Author information

  • 1Stowers Institute for Medical Research, Kansas City, Missouri, USA.

Abstract

Set2-mediated methylation of histone H3 Lys36 (H3K36) is a mark associated with the coding sequences of actively transcribed genes, but it has a negative role during transcription elongation. It prevents trans-histone exchange over coding regions and signals for histone deacetylation in the wake of RNA polymerase II (RNAPII) passage. We have found that in Saccharomyces cerevisiae the Isw1b chromatin-remodeling complex is specifically recruited to open reading frames (ORFs) by H3K36 methylation through the PWWP domain of its Ioc4 subunit in vivo and in vitro. Isw1b acts in conjunction with Chd1 to regulate chromatin structure by preventing trans-histone exchange from taking place over coding regions. In this way, Isw1b and Chd1 are important in maintaining chromatin integrity during transcription elongation by RNAPII.

PMID:
22922743
[PubMed - indexed for MEDLINE]
PMCID:
PMC3560298
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Write to the Help Desk