Display Settings:


Send to:

Choose Destination
Methods Mol Biol. 2012;916:141-55. doi: 10.1007/978-1-61779-980-8_11.

Labeling primitive myeloid progenitor cells in Xenopus.

Author information

  • 1The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester, UK.


In Xenopus the first blood cells to differentiate in the embryo are the primitive myeloid lineages, which arise from the anterior ventral blood islands during the neurula stages. Primitive myeloid cells (PMCs) will give rise to the embryonic pool of neutrophils and macrophages, a highly migratory population of cells with various functions during development and tissue repair. Understanding the development and behavior of PMCs depends on our ability to label, manipulate, and image these cells. Xenopus embryos have several advantages in the study of PMCs, including a well-established fate map and the possibility of performing transplants in order to label these cells. In addition, Xenopus embryos are easy to manipulate and their external development and transparency at the tadpole stages make them amenable to imaging techniques. Here we describe two methods for labeling primitive myeloid progenitor cells during early Xenopus development.

[PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Icon for Springer
    Loading ...
    Write to the Help Desk