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Arterioscler Thromb Vasc Biol. 2012 Oct;32(10):2372-9. doi: 10.1161/ATVBAHA.111.236158. Epub 2012 Aug 16.

Endogenous renovascular hypertension combined with low shear stress induces plaque rupture in apolipoprotein E-deficient mice.

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  • 1Department of Cardiology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Abstract

OBJECTIVE:

The development of a murine model of spontaneous atherosclerotic plaque rupture with luminal thrombus.

METHODS AND RESULTS:

Combined partial ligation of the left renal artery and left common carotid artery in 8-week-old apolipoprotein E-deficient mice induced endogenous renovascular hypertension and local low oscillatory shear stress in the left common carotid artery. After 8 weeks, a fresh left common carotid artery lumen thrombus associated with severe plaque burden was found in 50% (10/20) of the mice. Histological analyses indicated that all left common carotid artery lesions had vulnerable features, and 50% (5/10) of the mice showed plaque rupture with a lumen thrombus. Multiple layers with layering discontinuity and intraplaque hemorrhages were found in 80% (8/10) of the mice. Further experiments showed that both increased blood pressure, and angiotensin-II contributed to plaque progression and vulnerability. Decreased intimal collagen associated with increased collagenase activity and matrix metalloproteinase expression also resulted in plaque disruption.

CONCLUSIONS:

We demonstrate a murine model of spontaneous plaque rupture with a high incidence of luminal thrombus. The model not only nicely recapitulates the pathophysiological processes of human plaque rupture but it is also simple, fast, and highly efficient to generate.

PMID:
22904273
[PubMed - indexed for MEDLINE]
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