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Cell Host Microbe. 2012 Aug 16;12(2):233-45. doi: 10.1016/j.chom.2012.06.008.

An atlas of the Epstein-Barr virus transcriptome and epigenome reveals host-virus regulatory interactions.

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  • 1Computational Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.

Abstract

Epstein-Barr virus (EBV), which is associated with multiple human tumors, persists as a minichromosome in the nucleus of B lymphocytes and induces malignancies through incompletely understood mechanisms. Here, we present a large-scale functional genomic analysis of EBV. Our experimentally generated nucleosome positioning maps and viral protein binding data were integrated with over 700 publicly available high-throughput sequencing data sets for human lymphoblastoid cell lines mapped to the EBV genome. We found that viral lytic genes are coexpressed with cellular cancer-associated pathways, suggesting that the lytic cycle may play an unexpected role in virus-mediated oncogenesis. Host regulators of viral oncogene expression and chromosome structure were identified and validated, revealing a role for the B cell-specific protein Pax5 in viral gene regulation and the cohesin complex in regulating higher order chromatin structure. Our findings provide a deeper understanding of latent viral persistence in oncogenesis and establish a valuable viral genomics resource for future exploration.

Copyright © 2012 Elsevier Inc. All rights reserved.

PMID:
22901543
[PubMed - indexed for MEDLINE]
PMCID:
PMC3424516
Free PMC Article

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