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J Endod. 2012 Sep;38(9):1239-43. doi: 10.1016/j.joen.2012.06.010. Epub 2012 Jul 10.

Decalcifying effect of different ethylenediaminetetraacetic acid irrigating solutions and tetraclean on root canal dentin.

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  • 1Department of Operative Dentistry, University of Pavia, Pavia, Italy.



The objective of this study was to evaluate and compare in vitro the decalcifying capability on root canal dentin of 4 different irrigating solutions.


Twenty-five freshly extracted maxillary central incisors were selected. The canals were prepared to obtain a total of 4 samples from each root. The samples were randomly divided into 5 equal experimental groups (n = 20) according to the irrigating solutions tested (Tetraclean, Largal Ultra, 17% ethylenediaminetetraacetic acid, Tubuliclean). For each irrigating solution, the specimens were immersed for 5, 10, and 15 minutes. After exposures, the concentration of Ca(2+) extracted was measured by inductively coupled plasma-atomic emission spectrometry. Data were analyzed by means of nonparametric tests, and statistical analysis was performed.


The higher release of Ca(2+) was observed in samples treated with Tetraclean; this citric acid-based agent showed significantly higher decalcifying capability. For all irrigating solutions tested, 10 minutes of application are sufficient to obtain the maximum Ca(2+) release.


The results indicated that Tetraclean could be applied to obtain more efficient removal of the smear layer and to facilitate the biomechanical instrumentation procedures.

Copyright © 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

[PubMed - indexed for MEDLINE]
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