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Nucleic Acids Res. 2012 Oct;40(19):9897-902. doi: 10.1093/nar/gks746. Epub 2012 Aug 9.

Quantitative mass spectrometry and PAR-CLIP to identify RNA-protein interactions.

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  • 1Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.

Abstract

Systematic analysis of the RNA-protein interactome requires robust and scalable methods. We here show the combination of two completely orthogonal, generic techniques to identify RNA-protein interactions: PAR-CLIP reveals a collection of RNAs bound to a protein whereas SILAC-based RNA pull-downs identify a group of proteins bound to an RNA. We investigated binding sites for five different proteins (IGF2BP1-3, QKI and PUM2) exhibiting different binding patterns. We report near perfect agreement between the two approaches. Nevertheless, they are non-redundant, and ideally complement each other to map the RNA-protein interaction network.

PMID:
22885304
[PubMed - indexed for MEDLINE]
PMCID:
PMC3479200
Free PMC Article

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