Electrostatic-spray ionization mass spectrometry

Anal Chem. 2012 Sep 4;84(17):7422-30. doi: 10.1021/ac301332k. Epub 2012 Aug 20.

Abstract

An electrostatic-spray ionization (ESTASI) method has been used for mass spectrometry (MS) analysis of samples deposited in or on an insulating substrate. The ionization is induced by a capacitive coupling between an electrode and the sample. In practice, a metallic electrode is placed close to but not in direct contact with the sample. Upon application of a high voltage pulse to the electrode, an electrostatic charging of the sample occurs leading to a bipolar spray pulse. When the voltage is positive, the bipolar spray pulse consists first of cations and then of anions. This method has been applied to a wide range of geometries to emit ions from samples in a silica capillary, in a disposable pipet tip, in a polymer microchannel, or from samples deposited as droplets on a polymer plate. Fractions from capillary electrophoresis were collected on a polymer plate for ESTASI MS analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin I / analysis
  • Anions / chemistry
  • Cations / chemistry
  • Electrodes
  • Polymers / chemistry
  • Spectrometry, Mass, Electrospray Ionization / instrumentation
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Static Electricity

Substances

  • Anions
  • Cations
  • Polymers
  • Angiotensin I