Send to:

Choose Destination
See comment in PubMed Commons below
J Bioinform Comput Biol. 2012 Dec;10(6):1231002. doi: 10.1142/S0219720012310026. Epub 2012 Aug 7.

Tutorial on de novo peptide sequencing using MS/MS mass spectrometry.

Author information

  • 1Department of Computer Science, National University of Singapore, 3 Science Drive 2, Singapore 117543, Singapore.


This paper is a self-contained introductory tutorial on the problem in proteomics known as peptide sequencing using tandem mass spectrometry. This tutorial deals specifically with de novo sequencing methods (as opposed to database search methods). We first give an introduction to peptide sequencing, its importance and history and some background on proteins. Next we show the relationship between a peptide and the final spectrum produced from a tandem mass spectrometer, together with a description of the various sources of complications that arise during the process of generating the mass spectrum. From there we model the computational problem of de novo peptide sequencing, which is basically the reverse problem of identifying the peptide which produced the spectrum. We then present several major approaches to solve it (including reviewing some of the current algorithms in each approach), and also discuss related problems and post-processing approaches.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Atypon
    Loading ...
    Write to the Help Desk