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J Am Chem Soc. 2012 Aug 22;134(33):13535-7. doi: 10.1021/ja303883c. Epub 2012 Aug 13.

The innate immune protein Nod2 binds directly to MDP, a bacterial cell wall fragment.

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  • 1Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA.


Mammalian Nod2 is an intracellular protein that is implicated in the innate immune response to the bacterial cell wall and is associated with the development of Crohn's disease, Blau syndrome, and gastrointestinal cancers. Nod2 is required for an immune response to muramyl dipeptide (MDP), an immunostimulatory fragment of bacterial cell wall, but it is not known whether MDP binds directly to Nod2. We report the expression and purification of human Nod2 from insect cells. Using novel MDP self-assembled monolayers (SAMs), we provide the first biochemical evidence for a direct, high-affinity interaction between Nod2 and MDP.

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