Format

Send to:

Choose Destination
See comment in PubMed Commons below
Int J Mol Sci. 2012;13(6):7607-16. doi: 10.3390/ijms13067607. Epub 2012 Jun 20.

Ultrasound-Assisted Extraction of Syringin from the Bark of Ilex rotunda Thumb Using Response Surface Methodology.

Author information

  • 1College of Pharmacy, Hebei University, Baoding 071002, China; E-Mails: hyzlc@126.com (L.-C.Z.); xhxiagx@126.com (X.-H.X.).

Abstract

In this work, a rapid extraction method based on ultrasound-assisted extraction (UAE) of syringin from the bark of Ilex rotunda Thumb using response surface methodology (RSM) is described. The syringin was analyzed and quantified by high performance liquid chromatography coupled with UV detection (HPLC-UV). The extraction solvent, extraction temperature and extraction time, the three main factors for UAE, were optimized with Box-Behnken design (BBD) to obtain the highest extraction efficiency. The optimal conditions were the use of a sonication frequency of 40 kHz, 65% methanol as the solvent, an extraction time of 30 min and an extraction temperature of 40 °C. Using these optimal conditions, the experimental values agreed closely with the predicted values. The analysis of variance (ANOVA) indicated a high goodness of model fit and the success of the RSM method for optimizing syringin extraction from the bark of I. rotunda.

KEYWORDS:

Box-Behnken design; Ilex rotunda; response surface methodology; syringin; ultrasound-assisted extraction

PMID:
22837716
[PubMed - in process]
PMCID:
PMC3397548
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
    Loading ...
    Write to the Help Desk