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Virology. 2012 Oct 25;432(2):444-51. doi: 10.1016/j.virol.2012.06.027. Epub 2012 Jul 24.

Perinuclear localization of the HIV-1 regulatory protein Vpr is important for induction of G2-arrest.

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  • 1Institute of Virology, University of Erlangen-Nuremberg, Erlangen 91054, Germany. Stefan.Soergel@viro.med.uni-erlangen.de

Abstract

The HIV-1 accessory protein Vpr induces G2 cell cycle arrest and apoptosis. Previous studies indicate that the induction of G2-arrest requires the localization of Vpr to the nuclear envelope. Here we show that treatment of Vpr-expressing HeLa cells with the caspase 3 inhibitor Z-DEVD-fmk induced accumulation of Vpr at the nuclear lamina, while other proteins or structures of the nuclear envelope were not influenced. Furthermore, Z-DEVD-fmk enhances the Vpr-mediated G2-arrest that even occurred in HIV-1(NL4-3)-infected T-cells. Mutation of Pro-35, which is important for the integrity of helix-α1 in Vpr, completely abrogated the Z-DEVD-fmk-mediated accumulation of Vpr at the nuclear lamina and the enhancement of G2-arrest. As expected, inhibition of caspase 3 reduced the induction of apoptosis by Vpr. Taken together, we could show that besides its role in Vpr-mediated apoptosis induction caspase 3 influences the localization of Vpr at the nuclear envelope and thereby augments the Vpr-induced G2-arrest.

Copyright © 2012 Elsevier Inc. All rights reserved.

PMID:
22832123
[PubMed - indexed for MEDLINE]
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